Leaf-associated bacteria from transgenic white poplar producing resveratrol-like compounds: isolation, molecular characterization, and evaluation of oxidative stress tolerance.
Identifieur interne : 003573 ( Main/Exploration ); précédent : 003572; suivant : 003574Leaf-associated bacteria from transgenic white poplar producing resveratrol-like compounds: isolation, molecular characterization, and evaluation of oxidative stress tolerance.
Auteurs : Alma Balestrazzi [Italie] ; Martina Bonadei ; Cinzia Calvio ; Fulvio Mattivi ; Daniela CarboneraSource :
- Canadian journal of microbiology [ 1480-3275 ] ; 2009.
Descripteurs français
- KwdFr :
- Bactéries (effets des médicaments et des substances chimiques), Bactéries (effets des radiations), Bactéries (génétique), Bactéries (isolement et purification), Données de séquences moléculaires (MeSH), Feuilles de plante (génétique), Feuilles de plante (microbiologie), Feuilles de plante (métabolisme), Phénomènes physiologiques bactériens (MeSH), Populus (génétique), Populus (microbiologie), Populus (métabolisme), Rayons ultraviolets (MeSH), Resvératrol (MeSH), Stilbènes (métabolisme), Stilbènes (pharmacologie), Stress oxydatif (MeSH), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (microbiologie), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- effets des médicaments et des substances chimiques : Bactéries.
- effets des radiations : Bactéries.
- génétique : Bactéries, Feuilles de plante, Populus, Végétaux génétiquement modifiés.
- isolement et purification : Bactéries.
- microbiologie : Feuilles de plante, Populus, Végétaux génétiquement modifiés.
- métabolisme : Feuilles de plante, Populus, Stilbènes, Végétaux génétiquement modifiés.
- pharmacologie : Stilbènes.
- Données de séquences moléculaires, Phénomènes physiologiques bactériens, Rayons ultraviolets, Resvératrol, Stress oxydatif.
English descriptors
- KwdEn :
- Bacteria (drug effects), Bacteria (genetics), Bacteria (isolation & purification), Bacteria (radiation effects), Bacterial Physiological Phenomena (MeSH), Molecular Sequence Data (MeSH), Oxidative Stress (MeSH), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Leaves (microbiology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Plants, Genetically Modified (microbiology), Populus (genetics), Populus (metabolism), Populus (microbiology), Resveratrol (MeSH), Stilbenes (metabolism), Stilbenes (pharmacology), Ultraviolet Rays (MeSH).
- MESH :
- chemical , metabolism : Stilbenes.
- chemical , pharmacology : Stilbenes.
- chemical : Resveratrol.
- drug effects : Bacteria.
- genetics : Bacteria, Plant Leaves, Plants, Genetically Modified, Populus.
- isolation & purification : Bacteria.
- metabolism : Plant Leaves, Plants, Genetically Modified, Populus.
- microbiology : Plant Leaves, Plants, Genetically Modified, Populus.
- radiation effects : Bacteria.
- Bacterial Physiological Phenomena, Molecular Sequence Data, Oxidative Stress, Ultraviolet Rays.
Abstract
The aim of this study was the isolation and characterization of the culturable bacteria inhabiting the leaves of transgenic white poplars (Populus alba L. 'Villafranca') engineered with the StSy gene for the production of resveratrol-like compounds. Resveratrol glucosides are available in small amounts from natural sources or by expensive chemical synthesis procedures. An alternative approach for the large-scale production of these relevant pharmaceuticals is the use of transgenic plants as bioreactors, although the occurrence of novel molecules in plants growing under field conditions might interfere, to some extent, with the associated microbial population. Both epiphytes and endophytes were isolated from the leaves of 2 StSy transgenic lines producing resveratrol glucosides and from an untransformed plant line grown in a greenhouse. Eleven isolates were recovered and classified as members of the genus Bacillus by 16S rDNA-based analysis. In addition, 2 isolates were classified as members of the Curtobacterium and Kocuria genera, respectively. Tolerance to hydrogen peroxide, UV-C, and paraquat was evaluated, as were the swimming and swarming motility of the leaf-associated bacteria. Interestingly, the isolates recovered from transgenic tissues showed the ability to withstand oxidative stress compared with isolates recovered from the untransformed poplar line. In vitro bioassays showed that trans-resveratrol inhibited both the swarming and swimming motilities in all the tested bacteria. The effects of trans-resveratrol on flagellin production, required for motility, were also investigated by immunoblot analysis.
DOI: 10.1139/w09-038
PubMed: 19767855
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Leaf-associated bacteria from transgenic white poplar producing resveratrol-like compounds: isolation, molecular characterization, and evaluation of oxidative stress tolerance.</title><author><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Genetics and Microbiology, University of Pavia, via Ferrata 1, 27100, Pavia, Italy. almbal04@unipv.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Genetics and Microbiology, University of Pavia, via Ferrata 1, 27100, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Bonadei, Martina" sort="Bonadei, Martina" uniqKey="Bonadei M" first="Martina" last="Bonadei">Martina Bonadei</name></author><author><name sortKey="Calvio, Cinzia" sort="Calvio, Cinzia" uniqKey="Calvio C" first="Cinzia" last="Calvio">Cinzia Calvio</name></author><author><name sortKey="Mattivi, Fulvio" sort="Mattivi, Fulvio" uniqKey="Mattivi F" first="Fulvio" last="Mattivi">Fulvio Mattivi</name></author><author><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19767855</idno><idno type="pmid">19767855</idno><idno type="doi">10.1139/w09-038</idno><idno type="wicri:Area/Main/Corpus">003457</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003457</idno><idno type="wicri:Area/Main/Curation">003457</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003457</idno><idno type="wicri:Area/Main/Exploration">003457</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Leaf-associated bacteria from transgenic white poplar producing resveratrol-like compounds: isolation, molecular characterization, and evaluation of oxidative stress tolerance.</title><author><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Genetics and Microbiology, University of Pavia, via Ferrata 1, 27100, Pavia, Italy. almbal04@unipv.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Genetics and Microbiology, University of Pavia, via Ferrata 1, 27100, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Bonadei, Martina" sort="Bonadei, Martina" uniqKey="Bonadei M" first="Martina" last="Bonadei">Martina Bonadei</name></author><author><name sortKey="Calvio, Cinzia" sort="Calvio, Cinzia" uniqKey="Calvio C" first="Cinzia" last="Calvio">Cinzia Calvio</name></author><author><name sortKey="Mattivi, Fulvio" sort="Mattivi, Fulvio" uniqKey="Mattivi F" first="Fulvio" last="Mattivi">Fulvio Mattivi</name></author><author><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name></author></analytic><series><title level="j">Canadian journal of microbiology</title><idno type="eISSN">1480-3275</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (drug effects)</term><term>Bacteria (genetics)</term><term>Bacteria (isolation & purification)</term><term>Bacteria (radiation effects)</term><term>Bacterial Physiological Phenomena (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Oxidative Stress (MeSH)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (microbiology)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Plants, Genetically Modified (microbiology)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Populus (microbiology)</term><term>Resveratrol (MeSH)</term><term>Stilbenes (metabolism)</term><term>Stilbenes (pharmacology)</term><term>Ultraviolet Rays (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bactéries (effets des médicaments et des substances chimiques)</term><term>Bactéries (effets des radiations)</term><term>Bactéries (génétique)</term><term>Bactéries (isolement et purification)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (microbiologie)</term><term>Feuilles de plante (métabolisme)</term><term>Phénomènes physiologiques bactériens (MeSH)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term><term>Populus (métabolisme)</term><term>Rayons ultraviolets (MeSH)</term><term>Resvératrol (MeSH)</term><term>Stilbènes (métabolisme)</term><term>Stilbènes (pharmacologie)</term><term>Stress oxydatif (MeSH)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (microbiologie)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Stilbenes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Stilbenes</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Resveratrol</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bactéries</term><term>Feuilles de plante</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term><term>Stilbènes</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Stilbènes</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Physiological Phenomena</term><term>Molecular Sequence Data</term><term>Oxidative Stress</term><term>Ultraviolet Rays</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Phénomènes physiologiques bactériens</term><term>Rayons ultraviolets</term><term>Resvératrol</term><term>Stress oxydatif</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was the isolation and characterization of the culturable bacteria inhabiting the leaves of transgenic white poplars (Populus alba L. 'Villafranca') engineered with the StSy gene for the production of resveratrol-like compounds. Resveratrol glucosides are available in small amounts from natural sources or by expensive chemical synthesis procedures. An alternative approach for the large-scale production of these relevant pharmaceuticals is the use of transgenic plants as bioreactors, although the occurrence of novel molecules in plants growing under field conditions might interfere, to some extent, with the associated microbial population. Both epiphytes and endophytes were isolated from the leaves of 2 StSy transgenic lines producing resveratrol glucosides and from an untransformed plant line grown in a greenhouse. Eleven isolates were recovered and classified as members of the genus Bacillus by 16S rDNA-based analysis. In addition, 2 isolates were classified as members of the Curtobacterium and Kocuria genera, respectively. Tolerance to hydrogen peroxide, UV-C, and paraquat was evaluated, as were the swimming and swarming motility of the leaf-associated bacteria. Interestingly, the isolates recovered from transgenic tissues showed the ability to withstand oxidative stress compared with isolates recovered from the untransformed poplar line. In vitro bioassays showed that trans-resveratrol inhibited both the swarming and swimming motilities in all the tested bacteria. The effects of trans-resveratrol on flagellin production, required for motility, were also investigated by immunoblot analysis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19767855</PMID><DateCompleted><Year>2009</Year><Month>11</Month><Day>09</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1480-3275</ISSN><JournalIssue CitedMedium="Internet"><Volume>55</Volume><Issue>7</Issue><PubDate><Year>2009</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Canadian journal of microbiology</Title><ISOAbbreviation>Can J Microbiol</ISOAbbreviation></Journal><ArticleTitle>Leaf-associated bacteria from transgenic white poplar producing resveratrol-like compounds: isolation, molecular characterization, and evaluation of oxidative stress tolerance.</ArticleTitle><Pagination><MedlinePgn>829-40</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1139/w09-038</ELocationID><Abstract><AbstractText>The aim of this study was the isolation and characterization of the culturable bacteria inhabiting the leaves of transgenic white poplars (Populus alba L. 'Villafranca') engineered with the StSy gene for the production of resveratrol-like compounds. Resveratrol glucosides are available in small amounts from natural sources or by expensive chemical synthesis procedures. An alternative approach for the large-scale production of these relevant pharmaceuticals is the use of transgenic plants as bioreactors, although the occurrence of novel molecules in plants growing under field conditions might interfere, to some extent, with the associated microbial population. Both epiphytes and endophytes were isolated from the leaves of 2 StSy transgenic lines producing resveratrol glucosides and from an untransformed plant line grown in a greenhouse. Eleven isolates were recovered and classified as members of the genus Bacillus by 16S rDNA-based analysis. In addition, 2 isolates were classified as members of the Curtobacterium and Kocuria genera, respectively. Tolerance to hydrogen peroxide, UV-C, and paraquat was evaluated, as were the swimming and swarming motility of the leaf-associated bacteria. Interestingly, the isolates recovered from transgenic tissues showed the ability to withstand oxidative stress compared with isolates recovered from the untransformed poplar line. In vitro bioassays showed that trans-resveratrol inhibited both the swarming and swimming motilities in all the tested bacteria. The effects of trans-resveratrol on flagellin production, required for motility, were also investigated by immunoblot analysis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Balestrazzi</LastName><ForeName>Alma</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Genetics and Microbiology, University of Pavia, via Ferrata 1, 27100, Pavia, Italy. almbal04@unipv.it</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bonadei</LastName><ForeName>Martina</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Calvio</LastName><ForeName>Cinzia</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Mattivi</LastName><ForeName>Fulvio</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Carbonera</LastName><ForeName>Daniela</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Can J Microbiol</MedlineTA><NlmUniqueID>0372707</NlmUniqueID><ISSNLinking>0008-4166</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013267">Stilbenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>Q369O8926L</RegistryNumber><NameOfSubstance UI="D000077185">Resveratrol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018407" MajorTopicYN="Y">Bacterial Physiological Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000077185" MajorTopicYN="N">Resveratrol</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013267" MajorTopicYN="N">Stilbenes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014466" MajorTopicYN="N">Ultraviolet Rays</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>9</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>9</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19767855</ArticleId><ArticleId IdType="pii">w09-038</ArticleId><ArticleId IdType="doi">10.1139/w09-038</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Bonadei, Martina" sort="Bonadei, Martina" uniqKey="Bonadei M" first="Martina" last="Bonadei">Martina Bonadei</name><name sortKey="Calvio, Cinzia" sort="Calvio, Cinzia" uniqKey="Calvio C" first="Cinzia" last="Calvio">Cinzia Calvio</name><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name><name sortKey="Mattivi, Fulvio" sort="Mattivi, Fulvio" uniqKey="Mattivi F" first="Fulvio" last="Mattivi">Fulvio Mattivi</name></noCountry><country name="Italie"><noRegion><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003573 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003573 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19767855
|texte= Leaf-associated bacteria from transgenic white poplar producing resveratrol-like compounds: isolation, molecular characterization, and evaluation of oxidative stress tolerance.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19767855" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |